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Genetic engineering and protein biochemistry - CIGEx

​ Cellule d'Ingéniérie Génétique et d'Expression - CIGEx
Published on 5 May 2025

​​The Cellule d’Ingenierie Gen​etique et d’Expression (CIGEx) is open to national academic scientific community. The CIGEx platform is part of the Paris-Saclay Genomics platforms network (GENOPS).

        

​The CIGEx offers services ranging from (1) molecular biology for cloning genes of interest, mutagenesis and genetic engineering; (2) protein biochemistry (expression in different systems, interaction tests, purification of recombinant proteins); (3) design and cloning of gRNAs for CRISPR/Cas9 experiments; (4) preparation of libraries for high-throughput sequencing; and (5) lentivirus production.

The CIGEx has been implemented with the goals ​​​​of :

  • providing efficient tools and technologies with well established know-how,
  • providing LIMS and quality control at all steps,
  • providing scientific and technological environment for users,
  • providing training for students and researchers​.


​Didier BUSSO
Principal investigator
Phone : + 33 (0)1 46 54 91 42
[email protected]

Secretary
Aurélie GOURET
Phone : +33 (0)1 46 54 98 66
[email protected]




Services
  • Molecular cloning of genes of interest using different technologies (GatewayTM, SLIC, Gibson-CloningTM, Restriction-ligation).
  • ​Genetic engineering of plasmids.
  • Design and cloning of gRNAs for CRISPR/Cas9 experiments.
  • Performing single-cell RNA-seq experiments and preparing the corresponding libraries.
  • Production of recombinant proteins in bacterial and baculovirus-infected insect cell expression systems.
  • Screening of expression, co-expression and solubility conditions.
  • Interaction tests by “pull-down” (IMAC, GST) or by immuno-precipitations.
  • Expression, co-expression and purification of recombinant proteins.
  • Lentivirus production.​
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Recent production

  • Cloning of protein-coding genes from different organisms (human, mouse, yeast, etc.).​
  • Cloning and mutation of various genes whose products are involved in fundamental cellular functions.
  • Cloning of shRNAs and genes into appropriate lentiviruses.
  • Expression and purification of proteins involved in the processes of DNA repair, replication and transcription, particularly in humans.
  • Genetic engineering of numerous cloning or expression vectors.​



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